Emily Kwan

Lab Manager

 

  • 2010-2018 Senior Research Technician, Lab Manager, Withers Lab (NCE), UBC
  • 1986-2010 Senior Research Technician, Research Associate, Cellulase Lab,    Department of Microbiology and Immunology, UBC
  • 1981-1986 Biochemist, Helix Biotech Ltd., Richmond, BC
  • 1982 Sc. Biochemistry, Simon Fraser University, Burnaby, BC

 

INTERESTS

Molecular biology; protein and enzymes.  Methods and techniques.

 

PUBLICATIONS

Goddard-Borger ED, Fiege B, Kwan EM, Withers SG.  “Glycosynthase-Mediated Assembly of Xylanase Substrates and Inhibitors” (2011) ChemBioChem 12, 1703-1711.

Schubert M, Poon DK, Wicki J, Tarling CA, Kwan EM, Nielsen JE, Withers SG, McIntosh LP. “Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: histidine characterization by NMR spectroscopy” (2007) Biochemistry 46, 7383-95.

Poon DK, Ludwiczek ML, Schubert M, Kwan EM, Withers SG, McIntosh LP. “NMR spectroscopic characterization of a beta-(1,4)-glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate” (2007) Biochemistry 46, 1759-70.

Kwan EM, Boraston AB, McLean BW, Kilburn DG, Warren RA. “N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation” (2005) Protein Eng Des Sel. 18, 497-501.

Gorenflo VM, pfeifer TA, Lesnicki G, Kwan EM, Grigliatti TA, Kilburn DG, Piret JM. “Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture” (2004) Cytotechnology 44, 93-102.

Boraston AB, Kwan E, Chiu P, Warren RA, Kilburn DG. “Recognition and hydrolysis of noncrystalline cellulose” (2003) J Bio Chem. 278, 6120-7.

Kwan E, Guarna MM, Boraston AB, Gilkes NR, Haynes CA, Kilburn DG, Warren RA. “Self-activating factor X derivative fused to the C-terminus of a cellulose-binding module: Production and properties” (2002) Biotechnology Bioengineering 79, 724-32.

Pfeifer TA, Guarna MM, Kwan EM, Lesnicki G, Theilmann DA, Grigliatti TA, Kilburn DG. “Expression analysis of a modified factor X in stably transformed insect cell lines” (2001) Protein Expr Purif 23, 233-41.

Guarna MM, Côté HC, Kwan EM, Rintoul GL, Meyhack B, Heim J, MacGillivray RT, Warren RA, Kilburn DG. “Factor X fusion proteins: improved production and use in the release in vitro of biologically active hirudin from an inactive alpha-factor-hirudin fusion protein” (2000) Protein Expr Purif. 20,133-41.

Tomme P, Kwan E, Gilkes NR, Kilburn DG, Warren RA. “Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities” (1996) J Bacteriol.178, 4216-23.

Meinke A, Damude HG, Tomme P, Kwan EM, Kilburn DG, Miller RC Jr, Warren RA, Gilkes NR. “Enhancement of the endo-beta-1,4-glucanase activity of an exocellobiohydrolase by deletion of a surface loop” (1995) J Biol Chem. 270, 4383-6.

Meinke A, Gilkes NR, Kwan EM, Kilburn DG, Warren RA, Miller RC Jr. “Cellobiohydrolase A (CbhA) from the cellulolytic bacterium Cellulomonas fimi is a beta-1,4-exocellobiohydrolase analogous to Trichoderma reesei CBH II” (1994) Mol Microbiol. 12, 413-22.

Bedarkar S, Gilkes NR, Kilburn DG, Kwan EM, Rose DR, Miller RC Jr, Warren RA, Withers SG. “Crystallization and preliminary X-ray diffraction analysis of the catalytic domain of Cex, an exo-beta-1,4-glucanase and beta-1,4-xylanase from the bacterium Cellulomonas fimi” (1992) J Mol Biol. 228, 693-5.