Kim, Y-W., Chen, H. and Withers, S. G. (2005) Carbohydr. Res. 340, 2735-41 “Enzymatic transglycosylation of xylose using a glycosynthase”. Pubmed
Lovering, A. L., Lee, S. S., Kim, Y. W., Withers, S. G. and Strynadka, N. C. (2005) J. Biol. Chem. 280, 2105-15 “Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate”. Pubmed
Hekmat, O, Kim, Y-W, Williams, S. J., He, S. and Withers, S. G. (2005) J. Biol. Chem. 280(42), 35126-35 “Active-site peptide ‘fingerprinting’ of glycosidases in complex mixtures by mass spectrometry: Discovery of a novel retaining b-1,4-glycanase in Cellulomonas fimi”. Pubmed
Duncan, G, Tarling, C. A., Bingle, W. H., Nomellini, J. F., Yamage, M, Dorocicz, I. R., Withers, S. G. and Smit, J. (2005) Appl. Biochem. Biotechnol. 127, 95-110 “Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus”. Pubmed
Knapp, S., Yang, C., Pabbaraja, S., Rempel, B., Reid, S. and Withers, S. G. (2005) J. Org. Chem. 70, 7715-20 “Synthesis and kinetic analysis of the N-acetylhexosaminidase inhibitor XylNAc-isofagomine”. Pubmed
Vocadlo, D. J. and Withers, S. G. (2005) Biochemistry 44, 12809-18 “Detailed comparative analysis of the catalytic mechanisms of b-N-acetylglucosaminidases from families 3 and 20 of glycoside hydrolases”. Pubmed
Xia, Z., Azurmendi, H. F., Lairson, L. L., Withers, S. G., Gabelli, S. B., Bianchet, M. A., Amzel, L. M. and Mildvan, A. S. (2005) Biochemistry 44, 8989-97 “Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction”. Pubmed
Müllegger, J., Jahn, M., Chen, H-M., Warren, R. A. J. and Withers, S. G. (2005) PEDS 18, 33-40 “Engineering of a thioglycoligase: randomized mutagenesis of the acid/base residue leads to the identification of improved catalysts”. Pubmed
Tauss, A., Greimel, P., Rupitz, K., Steiner, A. J., Stütz, A. E. Withers, S. G. and Wrodnigg, T. M. Tetrahedron: Asymmetry 16, 159-65 “Non-natural aldofuranosides as substrates of a b-glucosidase” (2005).
Rempel, B. P., Clarke, L. A. and Withers, S. G. (2005) Mol. Genet. Metab. 85, 28-37 “A homology model for human a-L-iduronidase: insights into human disease”. Pubmed
Varrot, A., Yip, V. Y. L., Li, Y., Rajan, S. S., Yang, X., Anderson, W., Thompson, J., Withers, S. G. and Davies, G. J. (2005) J. Mol. Biol. 346(2), 423-35 “NAD+ dependent hydrolysis by family 4 glycosidase: structural insight into specificity for b-D-glucosides”. Pubmed
Maurus, R., Begum, A. Kuo, H.-H., Racaza, A., Numao, S., Andersen, C., Tams, J. W., Vind, J., Overall, C. M., Withers, S. G. and Brayer, G. D. (2004) Protein Sci. 14, 743-55 “Structural and mechanistic studies of chloride induced activation of human pancreatic a-amylase”. Pubmed
Li, C., Begum, A., Numao, S., Park, K. H., Withers, S. G. and Brayer, G. D. (2005) Biochemistry 44, 3347-57 “Acarbose rearrangement mechanism implied by the kinetic and structural analysis of human pancreatic a-amylase in complex with analogues and their elongated counterparts”. Pubmed
Vocadlo, D. J. and Withers, S. G. (2005) Carbohydr. Res. 340, 379-88 “The synthesis of probes of the hen egg white lysozyme mechanism”. Pubmed
Tarling, C. A. and Withers, S. G. (2004) Carbohydr. Res. 339, 2487-97 “The synthesis of a series of modified manno-trisaccharides as probes of the enzymes involved in the early stages of mammalian complex N-glycan formation”. Pubmed
Lairson, L. L. and Withers, S. G. (2004) (Focus article) Chem. Commun. 2243–8 “Mechanistic analogies amongst carbohydrate modifying enzymes”. Pubmed
Gloster, T. M., Macdonald, J. M., Tarling, C. A., Stick, R. V., Withers, S. G. and Davies, G. J. (2004) J. Biol. Chem. 279, 49236-42 “Structural, thermodynamic and kinetic analysis of tetrahydrooxazine-derived inhibitors bound to b-glucosidases”. Pubmed
Kim, Y-W, Lee, S. S., Warren, R. A. J. and Withers, S. G. (2004) J. Biol. Chem. 279, 42787-93 “Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire”. Pubmed
Numao, S., Damager, I., Li, C., Wrodnigg, T. M., Begum, A., Overall, C. M., Brayer, G. D. and Withers, S. G. (2004) J. Biol. Chem. 279, 48282-91 “In situ extension as an approach for identifying novel a-amylase inhibitors”. Pubmed
Yip, V. L. Y. and Withers, S. G. (2004) Org. Biomol. Chem. 2, 2707-13 “Nature’s many mechanisms for degradation of oligosaccharides”. Pubmed
Gloster, T., Williams, S. J., Roberts, S., Tarling, C. A., Wicki , J., Withers, S. G. and Davies, G. J. (2004) Chem. Commun., 1794-5 “Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to Xyn10A”. Pubmed
Damager, I., Numao, S., Chen, H., Brayer, G. D. and Withers, S. G. (2004) Carbohydr. Res. 339, 1727-37 “Synthesis and characterization of novel chromogenic substrates for human pancreatic a-amylase”. Pubmed
Rajan, S. S., Yang, X., Collart, F., Yip, V., Withers, S. G., Varrot, A., Thompson, J., Davies, G. J. and Anderson, W. F. (2004) Structure 12, 1619-29 “NAD-dependent hydrolysis by Family 4 glycosidases involves a novel elimination mechanism”. Pubmed
Watts, A. G. and Withers, S. G. (2004) Can J. Chem 82, 1581-1589 “The synthesis of some mechanistic probes for sialic acid processing enzymes and the labeling of a sialidase from Trypanosoma rangeli”.
Yip, V. L. Y., Varrot, A., Davies, G. J., Rajan, S. S., Yang, X., Thompson, J., Anderson, W. F. and Withers, S. G. (2004) J. Amer. Chem. Soc. 126, 8354-8355 “An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a Family 4 b-glycosidase from Thermotoga maritima”. Pubmed
Watts, A. G. and Withers, S. G. (2004) Biochem. J. 380, e9-e10 “Glycosynthase-catalysed formation of modified polysaccharide microstructures”. Pubmed
Wrodnigg, T. M., Diness, F., Gruber, C., Häusler, H., Lundt, I., Rupitz, K., Steiner, A. J., Stütz, A. E., Tarling, C. A., Withers, S. G. and Wölfler, H. (2004) Bioorg. Med. Chem. 12, 3485-95 “Probing the aglycon binding site of a b-glucosidase: a library of C-1-modified 2,5-dideoxy-2,5-imino-D-mannitol derivatives and their structure-activity relationships”. Pubmed
Lairson, L. L, Chiu, C. P, Ly, H. D., He, S., Wakarchuk, W. W., Strynadka, N. C. J. and Withers, S. G. (2004) J. Biol. Chem. 279, 13478-87 “Intermediate trapping on a mutant retaining a-galactosyl transferase identifies an unexpected aspartate residue”. Pubmed
Amaya, M. F., Watts, A., Damager, I., Wehenkel, A., Nguyen, T., Buschiazzo, A., Paris, G., Frasch, A. C., Withers, S. G. and Alzari, P. M. (2004) Structure 12, 775-84 “Structural insights into the catalytic mechanism of Trypanosoma cruzi trans-sialidase”. Pubmed
Sulzenbacher, G., Bignon, C., Nishimura, T., Tarling, C. A., Withers, S. G., Henrissat, B. and Bourne, Y. (2004) J. Biol. Chem. 279, 13119-28 “Crystal structure of Thermotoga maritima a-L-fucosidase, TM0306: structural basis for catalytic mechanism and fucosidosis”. Pubmed
Rye, C. S. and Withers, S. G. (2004) Carbohydr. Res. 339, 699-703 “The synthesis of a novel thio-linked disaccharide of chondroitin as a potential inhibitor of chondroitin AC lyase from Flavobacterium heparinum”. Pubmed
Jahn, M., Chen, H., Muellegger, J., Marles, J., Warren, R. A. J. and Withers, S. G. (2004) JCS Chem. Commun. 3, 274-5 “Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis” Pubmed
Chiu, C. P. C., Watts, A., Lairson, L., Gilbert, M., Lim, D., Wakarchuk, W. W., Withers, S. G. and Strynadka, N. C. J. (2004) Nat. Struct. Biol. 11 , 163-70 “Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog”. Pubmed
Yang, J. K., Yoon, H-J, Ahn, H. J., Lee, B. I., Pedelacq, D., Liong, E. C., Berendzen, J., Laivenieks, M., Vieille, C., Zeikus, G. J., Vocadlo, D. J., Withers, S. G. and Suh, S. W. (2004) J. Mol Biol. 355, 155-65 “Crystal structure of b-D-xylosidase from Thermoanaerobacterium saccharolyticum, a glycoside hydrolase of family 39”. Pubmed
Gibson, R. P., Tarling, C. A., Roberts, S., Withers, S. G. and Davies, G. J. (2004) J Biol. Chem. 279, 1950-5 “The donor subsite of the trehalose-6-phosphate synthase OtsA: binary complexes with UDP-Glc and UDP-2fluoro glucoside at 2.1 Å resolution”. Pubmed
Tropak, M. B., Reid, S. P. , Guiral, M., Withers, S. G. and Mahuran, D. (2004) J. Biol. Chem 279, 13478-87 “Pharmacological enhancement of b-hexosaminidase activity in fibroblasts from Adult Tay-Sachs and Sandhoff patients”. Pubmed
Tarling, C. A., He, S., Sulzenbacher, G., Bignon, C., Bourne, Y., Henrissat, B. and Withers, S. G. (2003) J. Biol. Chem 278, 47394-9 “Identification of the catalytic nucleophile of the Family 29 -L-Fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis”. Pubmed
Numao, S., Kuntz, D. A., Withers, S. G. and Rose, D. R. (2003) J. Biol. Chem 278, 48074-83 “Insights into the mechanism of Drosophila melanogaster Golgi -mannosidase II through the structural analysis of covalent reaction intermediates”. Pubmed
Paal, K., Ito, M. and Withers, S. G. (2004) Biochem. J. 378, 141-9 “Paenibacillus sp. TS12 glucosylceramidase – Kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues”. Pubmed
Lee, S., Yu, S. and Withers, S. G. (2003) Biochemistry 42, 13081-90 “Detailed dissection of a new mechanism for glycoside cleavage: the a-1,4-glucan lyase”. Pubmed
Jahn, M. and Withers, S. G. (2003) Biocatal. Biotransform. 21, 159-66 “New approaches to enzymatic oligosaccharide synthesis: glycosynthases and thioglycoligases”.
Ducros, V. M-A, Tarling, C. A., Zechel, D. L., Brzozowski, A. M., Frandsen, T. P., von Ossowski, I., Schulein, M., Withers, S. G. and Davies, G. J. (2003) Chem. Biol.10, 619-28 “Anatomy of glycosynthesis: structure and kinetics of the Humicola insolens Cel7B E197A and E197S glycosynthase mutants”. Pubmed
Varrot, A., Tarling, C., MacDonald, J., Stick, R. V., Zechel, D. L., Withers, S. G. and Davies, G. J. (2003) J. Amer. Chem. Soc. 125, 7496-7 “Direct observation of the protonation state of an imino-sugar glycosidase inhibitor upon binding”. Pubmed
Zechel, D. L., Reid, S. P., Stoll, D., Nashiru, O., Warren, R. A. J and Withers, S. G. (2003) Biochemistry 42, 7195-204 “Mechanism, mutagenesis and chemical rescue of a b-Mannosidase from Cellulomonas fimi”. Pubmed
Jahn, M., Stoll, D., Warren, R. A. J., Szabo, L., Singh, P., Gilbert, H. J., Ducros, V. M. A., Davies, G. J. and Withers, S. G. (2003) JCS Chem. Commun. 12, 1327-9 “Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides”. Pubmed
Hekmat, O., Tokuyasu, K. and Withers, S. G. (2003) Biochem. J. 374, 369-80 “Subsite structure of the endo-type chitin deacetylase from a Deuteromycete, Colletotrichum lindemuthianum”: an investigation using steady-state kinetic analysis and MS”. Pubmed
Nieman, C. E., Wong, A. W., He, S., Clarke, L., Hopwood, J. J. and Withers, S. G. (2003) Biochemistry 42, 8054-65 “Family 39 a-L-Iduronidases and b-D-Xylosidases react through similar glycosyl-enzyme intermediates: identification of the human iduronidase nucleophile”. Pubmed
Watts, A., Damager, I., Amaya, M. L., Buschiazzo, A., Alzari, P., Frasch, A. C. and Withers, S. G. (2003) J. Amer. Chem. Soc. 125, 7532-3 “Trypanosoma cruzi trans-sialidase operates through a covalent sialyl-enzyme intermediate: tyrosine is the catalytic nucleophile”. Pubmed
Gloster, T., Williams, S. J. , Tarling, C. A., Roberts, S., Dupont, C., Jodoin, P., Shareck, F., Withers, S. G. and Davies, G. J. (2003) JCS Chem. Commun. 8, 944-5 “A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer”. Pubmed
Withers, S. G., Wakarchuk, W. W. and Strydnaka, N. C. J. (2002) Chem. & Biol. 9, 1270-3 “One step closer to a sweet conclusion”. Pubmed
Poon, D. K. Y., Webster, P., Withers, S. G. and McIntosh, L. P. (2003) Carbohydr. Res. 338, 415-21 “Characterizing the pH-dependent stability and catalytic mechanism of the family 11 xylanase from the alkalophilic Bacillus agaradherans”. Pubmed
Jahn, M., Marles, J, Warren, R.A.J. and Withers, S.G. (2003) Angew. Chem. Int. Ed. 115, 366-8 “Thioglycoligases: mutant glycosidases for thioglycoside synthesis”. Pubmed
Macdonald, J. A., Stick, R. V., Tilbrook, D. M. G. and Withers, S. G. (2002) Aus J. Chem. 55, 747-52 “Synthesis with glycosynthases: cello-oligomers of isofagomine and a tetrahydrooxazine as cellulase inhibitors”.
Williams, S. J., Mark, B., Vocadlo, D. J., James, M. N. G. and Withers, S. G. (2002) J. Biol. Chem. 277, 40055-65 “Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state”. Pubmed
Rye, C. and Withers, S. G. (2002) Anal. Biochem. 38, 77-82 “Development of an assay and determination of kinetic parameters for chondroitin AC lyase using defined synthetic substrates”. Pubmed
Rye, C. and Withers, S. G. (2002) J. Amer. Chem. Soc. 124, 9756-67 “Mechanistic studies of chondroitin AC lyase from Flavobacterium heparinum”. Pubmed
Chir, J., Withers, S. G., Wan, J-F. and Li, Y-K. (2002) Biochem. J. 365, 857-63 “Identification of the two essential groups in the family 3 b-glucosidase from Flavobacterium meningosepticum by labeling and tandem mass spectrometric analysis”.
Ducros, V., Zechel, D. L., Gilbert, H. J., Withers, S. G. and Davies, G. J. (2002) Angew. Chemie 41, 2824-7 “Substrate distortion by a b -mannanase: snapshots of the Michaelis and intermediate complexes suggest a B2,5 conformation for the transition state” Pubmed.
Hausler, H., Rupitz, K., Stuetz, A. and Withers, S. G. (2002) Monatsh. Chem. 133, 555-60 “N-Alkylated derivatives of 1,5-dideoxy-1,5-imino-D-xylitol as b-xylosidase and b-glucosidase inhibitors”.
Vocadlo, D. J., Wicki, J., Rupitz, K. and Withers, S. G. (2002) Biochemistry 41, 9736-46 “A case for reverse protonation: Identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum b-xylosidase and detailed kinetic analysis of a site-directed mutant”. Pubmed
Vocadlo, D. J., Wicki, J., Rupitz, K. and Withers, S. G. (2002) Biochemistry 41, 9727-35 “Mechanism of Thermoanaerobacterium saccharolyticum b-xylosidase: kinetic studies”. Pubmed
Tolborg, J. F., Petersen, L., Jensen, K. J., Mayer, Ch., Jakeman, D. L, Warren, R. A. J. and Withers, S. G. (2002) J. Org. Chem. 67, 4143-9 “Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases”. Pubmed
Rye, C. and Withers, S. G. (2002) J. Org. Chem. 67, 4505-12 “Synthesis and evaluation of potential inhibitors of chondroitin AC lyase from Flavobacterium heparinum”. Pubmed
Lee, S., He, S., Yu, S. and Withers, S. G. (2002) J. Amer. Chem. Soc. 124, 4948-9 “a-Glucan lyase catalyses a trans elimination via a nucleophilic displacement followed by a syn elimination”. Pubmed
Jakeman, D. L. and Withers, S. G. (2002) Can. J. Chem. 80, 866-70 “On expanding the synthetic repertoire of glycosynthases: mutant b-galactosidases forming b (1,6) linkages”.
Williams, S. J. and Withers, S. G. (2002) Aus. J. Chem. 55, 3-12 “Glycosynthases: mutant glycosidases for glycoside synthesis”.
Jakeman, D. and Withers, S. G. (2002) Trends in Glycoscience and Glycotechnology 14, 13-25 “Glycosynthases: new tools for oligosaccharide synthesis”.
Ly, H. D., Lougheed, B., Wakarchuk. W. W. and Withers, S. G. (2002) Biochemistry 41, 5075-85 “Mechanistic studies of a retaining a-galactosyltransferase from Neisseria meningitides”. Pubmed
Agnihotri, A., He, S., Hong, L., Dakoji, S., Withers, S. G. and Liu, H.-W. (2002) Biochemistry 41, 1843-52 “A revised mechanism for the inactivation of bovine liver enoyl-CoA hydratase by (Methylenecyclopropyl)formyl-CoA based on unexpected results with the C114A mutant”. Pubmed
Rydberg, E. H., Li, C., Maurus, R., Overall, C. M., Brayer, G. D. and Withers, S. G. (2002) Biochemistry 41, 4492-502 “Mechanistic analyses of catalysis in human pancreatic a-amylase: detailed kinetic and structural studies of mutants of three conserved carboxylic acids”. Pubmed
Zechel, D. L. and Withers, S. G. (2001) Curr. Opin. Chem. Biol. 5, 643-9 “Dissection of nucleophilic and acid/base catalysis in glycosidases”. Pubmed
Juers, D. H., Heightman, T. D., Vasella, A., McCarter, J. D., Mackenzie, L. F., Withers, S. G. and Matthews, B. W. (2002) Biochemistry 40, 14781-94 “A structural view of the action of E. coli (lac Z) b-galactosidase”. Pubmed
Numao, S., Maurus, R., Sidhu, G., Wang, Y., Overall, C. M., Brayer, G. D. and Withers, S. G. (2002) Biochemistry 41, 215-25 “Probing the role of the chloride ion in the mechanism of human pancreatic a-amylase”. Pubmed
Jakeman, D. and Withers, S.G. (2002) “Engineering glycosidases for constructive purposes” in ‘Carbohydrate Bioengineering: Interdisciplinary approaches’ Eds. T. Teeri, B. Svensson, H. J. Gilbert, T. Feizi, The Royal Society of Chemistry, Cambridge, U.K, pp 3-8.
Wicki, J., Rose, D. and Withers, S. G. (2002) Meths. Enzymol. 354, 84-105 “Trapping intermediates on b-glycosidases”. Pubmed
Mosi, R. and Withers, S. G. (2002) Meths. Enzymol. 354, 64-84 “Detection and characterization of intermediates on a-glycosyl transferases”. Pubmed
Mark, B., Vocadlo, D., Zhao, D., Knapp, S., Withers, S. G. and James, M. N. G. (2001) J. Biol. Chem. 276, 42131-7 “Biochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 b-N-acetylhexosaminidase inhibitor”. Pubmed
Vocadlo, D., Davies, G. J., Laine, R. and Withers, S. G. (2001) Nature 412, 835-8 “Catalysis by lysozyme proceeds via a covalent intermediate”. Pubmed
Lee, S., He, S. and Withers, S. G. (2001) Biochem. J. 359, 381-6 “Identification of the catalytic nucleophile of the Family 31 a-glucosidase from Aspergillus niger via trapping of a 5-fluoroglycosyl-enzyme intermediate”. Pubmed
Brun, E., Brumer III, H., Mackenzie, L. F., Withers, S. G. and McIntosh, L. P. (2001) J. Biomol. NMR 21, 67-8 “Assignment of a selectively 13C-labeled cellopentaose using an engineered glycosynthase”. Pubmed
Joshi, M., Sidhu, G, Nielsen J. E., Brayer, G. D., Withers, S.G. and McIntosh, L.P. (2001) Biochemistry 40, 10115-39 “Dissecting the electrostatic interactions and pH-dependent activity of a Family 11 glycosidase”. Pubmed
Hermetter, A., Scholze, H., Stutz, A. E., Withers, S. G. and Wrodnigg, T. M. (2001) Bioorg. Med. Chem. Lett. 11, 1339-42 “Powerful probes for glycosidases: novel, fluorescently tagged glycosidase inhibitors”. Pubmed
Blanchard, J., Gal, L., He, S., Warren, R. A. J. and Withers, S. G. (2001) Carbohydr. Res. 333, 7-17 “The identification of Glu260 and Glu233 as the catalytic nucleophiles of Family 35 b-galactosidases from Xanthomonas manihotis and Bacillus circulans”. Pubmed
Fairweather, J. K., Stick, R. V. and Withers, S. G. (2001) Aus. J. Chem. 53, 913-6 “A ‘Glycosynthase’-assisted synthesis of some epoxyalkyl b-C-oligosaccharides”.
Blanchard, J. and Withers, S. G. (2001) Chem.Biol. 8, 627-33 “Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides”. Pubmed
Wrodnigg, T. M., Withers, S. G. and Stutz, A. E. (2001) Bioorg. Med. Chem. Lett. 11, 1063-4 “Novel lipophilic derivatives of 2,5-dideoxy-2,5-imino-D-mannitol are powerful b-glucosidase inhibitors”. Pubmed
Mayer, C., Jakeman, D. L., Mah, M., Karjala, G., Gal, L., Warren, R. A. J. and Withers, S. G. (2001) Chem. Biol. 8, 437-43 “Screening for new and improved glycosynthases”. Pubmed
Persson, K., Ly, H., Dieckelmann, M., Wakarchuk, W. W., Withers, S. G. and Strynadka, N. (2001) Nat. Struct. Biol. 8, 166-75 “Crystal structure of the retaining galactosyl transferase from Neisseria meningitides in complex with donor and acceptor sugar analogues: towards a mechanism”. Pubmed
Wong, A. W., Adam, M. J. and Withers, S. G. (2001) J. Labelled Compd. Radiopharm. 44, 385-94 “Synthesis of 2,6-dideoxy-2-fluoro-6-[18F]- b-D-glucopyranosyl fluoride (2,5-FGF) as a potential imaging probe for glucocerebrosidase”.
Mark, B. L., Vocadlo, D. J., Triggs-Raine, B. L., Withers, S. G. and James, M. N. G. (2001) J. Biol. Chem. 276, 10330-7 “Insights into the mechanisms of retaining hexosaminidases: crystal structure of Streptomyces plicatus b-hexosaminidase in complex with the cyclic intermediate analogue NAG-thiazoline at 2.1 Å”. Pubmed
Zechel, D. L., Reid, S. P., Mayer, C., Stoll, D., Nashiru, O., Jakeman, D. L., Warren, R. A. J. and Withers, S. G. (2001) J. Amer. Chem. Soc. 123, 4350-1 “Enzymatic synthesis of carbon-fluorine bonds”. Pubmed
Nashiru, O., Zechel, D. L., Stoll, D., Mohammadzadeh, T., Warren, R. A. J. and Withers, S. G. (2001) Angew. Chem. Int. Ed. 40, 417-20 “b-Mannosynthase: high yielding enzymatic synthesis of b-mannoside linkages”. Pubmed
Hou, Y., Vocadlo, D., Leung, A., Withers, S. G. and Mahuran, D. (2001) Biochemistry 40, 2201-9 “Characterization of the Glu and Asp residues in the active site of human b-Hexosaminidase B”. Pubmed
Wong, A. W., He, S. and Withers, S. G. (2001) Can. J. Chem. 79, 510-8 “Synthesis of 5-fluoro-b-D-glucopyranosyluronic acid fluoride and its evaluation as a mechanistic probe of E. colib-glucuronidase”.