Goddard-Borger, E. D., Wennekes, T. and Withers, S. G. (2010) Nat. Chem. Biol. 6, 881-3 “Molecular probes: Getting lucky in the lysosome”. Pubmed
Kim, Y.-W., Zhang, R., Chen, H. and Withers, S. G. (2010) Chem. Commun. 46, 8725-7 “O-Glycoligases, a new category of glycoside bond-forming mutant glycosidases, catalyse facile syntheses of isoprimeverosides”. Pubmed HOT article
Chen, H.-M. and Withers, S. G. (2010) Carbohydr. Res. 345, 2596-604 “Syntheses of p-nitrophenyl 3- and 4-thio-β-D-glycopyranosides”. Pubmed
Kim, E. J., Love, D. C., Darout, E., Abdo, M., Rempel, B., Withers, S. G., Rablen, P. R., Hanover, J. A. and Knapp, S. (2010) Bioorg. Med. Chem. 18, 7058–64 “OGA inhibition by GLCNAc-selenazoline”. Pubmed
Schitter, G., Steiner, A. J., Pototschnig, G., Scheucher, E., Thonhofer, M., Tarling, C. A., Withers, S. G., Fantur, K., Paschke, E., Mahuran, D. J., Rigat, B. A., Tropak, M. B., Illaszewicz, C., Saf, R., Stuetz, A. E. and Wrodnigg, T. M. (2010) Chembiochem 11, 2026-33 “Fluorous iminoalditols: a new family of glycosidase inhibitors and pharmacological chaperones”. Pubmed
Yang, G., Rich, J. R., Gilbert, M., Wakarchuk, W. W., Feng, Y. and Withers, S. G. (2010) J. Am. Chem. Soc. 132, 10570-7 “Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases”. Pubmed
Pototschnig, G., De Csáky, C. M., Burke, J. R. M., Schitter, G., Stütz, A. E., Tarling, C. A., Withers S. G. and Wrodnigg, T. M. (2010) Bioorg. Med. Chem. Letts. 20, 4077-9 “Synthesis and biological evaluation of novel biotin-iminoalditol conjugates”. Pubmed
Morley, T. J. and Withers, S. G. (2010) J. Am. Chem. Soc. 132, 9430-7 “Chemoenzymatic synthesis and enzymatic analysis of 8-modified cytidine monophosphate-sialic acid and sialyl lactose derivatives”. Pubmed
Phenix, C. P., Rempel, B. P., Colobong, K., Doudet, D. J., Adam, M. J., Clarke, L. A. and Withers, S. G. (2010) Proc. Nat. Acad. Sci. U.S.A. 107, 10842-7 “Imaging of enzyme replacement therapy using PET”. Pubmed
Froehlich, R. F. G., Furneaux, R. H., Mahuran, D. J., Rigat, B. A., Stutz, A. E., Tropak, M. B., Wicki, J., Withers, S. G. and Wrodnigg, T. M. (2010) Carbohydr. Res. 345, 1371-6 “1-Deoxynojirimycins with dansyl capped N-substituents as probes for morbus Gaucher affected cell lines”. Pubmed
Kittl, R. and Withers, S. G. (2010) Carbohydr. Res. 345, 1272-9 “New approaches to enzymatic glycoside synthesis through directed evolution”. Pubmed
Reitinger, S., Yu, Y., Wicki, J., Ludwiczek, M., D’Angelo, I., Baturin, S., Okon, M., Strynadka, N. C. J., Lutz, S., Withers, S. G. and McIntosh, L. P. (2010) Biochemistry 49, 2464-74 “Circular permutation of Bacillus circulans xylanase: a kinetic and structural study”. Pubmed
Schitter, G., Scheucher, E., Steiner, A. J., Stuetz, A. E., Thonhofer, M., Tarling, C. A., Withers, S. G., Wicki, J., Fantur, K., Paschke, E., Mahuran, D. J., Rigat, B. A., Tropak, M. and Wrodnigg, T. M. (2010) Beilstein J. Org. Chem. 6, 21 “Synthesis of lipophilic 1-deoxygalactonojirimycin derivatives as D-galactosidase inhibitors”. Pubmed
Macdonald, J. M., Tarling, C. A., Taylor, E. J., Dennis, R. J., Myers, D. S., Knapp, S., Davies, G. J. and Withers, S. G. (2010) Angew. Chemie, Int. Ed. 49, 2599-602 “Chitinase inhibition by chitobiose and chitotriose thiazolines”. Pubmed
Armstrong, Z., Reitinger, S., Kantner, T. and Withers S. G. (2010) Chembiochem 11, 533-8 “Enzymatic thioxyloside synthesis: Characterization of thioglycoligase variants identified from a site-saturation mutagenesis library of Bacillus circulans xylanase”. Pubmed
Zhang, R., Yip, V., Withers, S. G. “Mechanisms of enzymatic glycosyl transfer” (2010) In Comprehensive Natural Products II Chemistry and Biology; Mander, L., Lui, H.-W, Eds.; Elsevier: Oxford, 2010; [Vol. 8], pp 385-422.
Willis, L., Zhang, R., Reid, A., Withers, S. G. and Wakarchuk, W. (2009) Biochemistry 48, 10334-41 “Mechanistic investigation of the endo-a-N-acetylgalactosaminidase from Streptococcus pneumoniae R6”. Pubmed
Zhang, R., Li, C., Williams, L. K., Rempel, B. P., Brayer, G. D. and Withers, S. G. (2009) Biochemistry 48, 10752-64 “Directed ‘in situ’ elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic a-amylase”. Pubmed
Chan, P. H. W., Lairson, L. L., Lee, H.-J. Wakarchuk, W. W., Strynadka, N. C. J., Withers, S. G. and McIntosh, L. P. (2009) Biochemistry 48, 11220-30 “NMR spectroscopic characterization of the sialyltransferase CstII from Campylobacter jejuni: Histidine 188 is the general base”. Pubmed
Yang, G. and Withers, S. G. (2009) Chembiochem 10, 2704-15 “Ultrahigh-throughput FACS-based screening for directed enzyme evolution”. Pubmed
Shaikh, F. A., Randriantsoa, M. and Withers, S. G. (2009) Biochemistry 48, 8396-404 “Mechanistic analysis of the blood group antigen-cleaving endo-galactosidase from Clostridium perfringens”. Pubmed
Rao, F. V., Rich, J. R., Rakic, B., Buddai, S., Schwartz, M., Johnson, K., Bowe, C., DeFrees, S., Wakarchuk, W. W., Withers, S. G. and Strynadka, N. C. J. (2009) Nature Struct. Mol. Biol. 16, 1186-8 “Structural insight into mechanism and specificity of mammalian sialyltransferases”. Pubmed
Kim, J. H., Ryu, H. W., Shim, J. H., Park, K. H. and Withers, S. G. (2009) Chembiochem 10, 2475-9 “Development of new and selective Trypanosoma cruzi trans-sialidase inhibitors from sulfonamide chalcones and their derivatives”. Pubmed
Lee, H. J., Rakic, B., Gilbert, M., Wakarchuk, W. W., Withers, S. G. and Strynadka, N. C. (2009) J. Biol. Chem. 284, 24501-11 “Structural and kinetic characterization of the polysialic acid O-acetyltransferase OatWY from Neisseria meningitides”. Pubmed
Morley, T. J., Willis, L. M., Whitfield, C., Wakarchuk, W. W. and Withers, S. G. (2009) J. Biol. Chem. 284, 17404-10 “A new sialidase mechanism: bacteriophage K1F endosialidase is an inverting glycosidase”. Pubmed
Rempel, B. P. and Withers S. G. (2009) Aus J. Chem. 62, 590-9 “Non-Stick sugars: synthesis and evaluation of difluorosugar fluorides as glycosidase inhibitors”.
Steiner, A. J., Stütz, A. E., Tarling, C. A., Withers, S. G. and Wrodnigg, T. M. (2009) Aus. J. Chem. 62, 553-7 “Synthesis and biological evaluation of 1,5-dideoxy-1,5-imino-d-xylitol – amino acid hybrids as xylosidase inhibitors”.
Offen, W. A., Zechel, D. L., Withers, S. G., Gilbert, H. J. and Davies, G. J. (2009) Chem. Commun. 18, 2484-6 “Structure of the Michaelis complex of β-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis”. Pubmed
Rakic, B. and Withers, S. G. (2009) Aus. J. Chem. 62, 510-20 “Recent developments in glycoside synthesis with glycosynthases and thioglycoligases”.
Hancock, S. M., Caines, M. E. C., Strynadka, N. C. J. and Withers, S. G. (2009) Nature Chem. Biol. 5, 508-14 “Designer enzymes for glycosphingolipid synthesis by directed evolution”. Pubmed
Steiner, A. J., Schitter, G., Stütz, A. E., Wrodnigg, T. M. Tarling, C. A., Withers, S. G., Mahuran, D. and Tropak, M. (2009) Tetrahedron: Asymmetry 20, 832–5 “2-Acetamino-1,2-dideoxynojirimycin – lysine hybrids as hexosaminidase inhibitors”. Pubmed
Davies, G. J, Withers, S. G. and Vocadlo, D. J. (2009) Aus. J. Chem. 62, 528-32 “The chitopentaose complex of a mutant hen-egg white lysozyme displays no distortion of the –1 sugar away from a 4C1 chair conformation”.
Rich, J. R. and Withers, S. G. (2009) Nature Chemical Biology 5, 206-15 “Emerging methods for the production of homogeneous human glycoproteins”. Pubmed
Aharoni, A. and Withers, S. G. “Screening methodologies for glycosidic bond formation” in the Protein Engineering Handbook, John Wiley. (2009) Vol. 2 605-20.
Steiner, A. J., Schitter, G., Stütz, A.,Wrodnigg, T.M, Tarling, C.A., Withers, S.G., Fantur, K., Mahuran, D., Paschke, E. and Tropak, M. (2008) Bioorg. Med. Chem. 16, 10216-20 “1-Deoxygalactonojirimycin-lysine hybrids as potent D-galactosidase inhibitors”. Pubmed
Greig, I. R., Zahariev, F. and Withers, S. G. (2008) J. Am. Chem. Soc. 130,17620-8 “Elucidating the nature of the Streptomyces plicatus b-hexosaminidase-bound intermediate using ab initio molecular dynamics simulations”. Pubmed
Le, Q.-T., Lee, C.-K., Kim, Y.-W., Lee, S.-J., Zhang, R., Withers, S. G., Kim, Y.-R., Auh, J.-H. and Park, K.-H. (2008) Carbohydr. Polym. 75, 9-14 “Amylolytically-resistant tapioca starch modified by combined treatment of branching enzyme and maltogenic amylase”.
Caines, M. E. C., Zhu, H., Vuckovic, M., Willis, L. M., Withers, S. G., Wakarchuk, W. W. and Strynadka, N. C. J. (2008) J. Biol. Chem. 283, 31279-83 “The structural basis for T-antigen hydrolysis by Streptococcus pneumoniae, a potential target for structure-based vaccine design”. Pubmed
Kuntz, D. A., Tarling, C. A, Stick, R. V., Withers, S. G. and Rose, D. R. (2008) Biochemistry 47, 10058–68 “Structural analysis of novel Golgi a-mannosidase II inhibitors from a focused glycosidase inhibitor screen”. Pubmed
Hancock, S., Tarling, C. and Withers S.G. (2008) Anal. Biochem. 382, 48-54 “High-throughput screening of cell lysates for ganglioside synthesis”. Pubmed
Kim, Y.-S., Lee, M.-H., Lee, H.-S., Lee, S.-J., Kim, Y.-W., Zhang, R, Withers, S. G., Kim, K.-S., Lee, S. -J. and Park, K.-H. (2008) J. Agric. Food Chem. 56, 5324-30 “Enzymatic synthesis of a selective inhibitor for glucosidases: acarviosinyl-(1-propenyl-1-deoxy-a-D-glucopyranoside)”. Pubmed
Eltis, L. D. and Withers, S. G. (2008) Curr. Opin. Chem. Biol. 12, 115-7 “Nature teaches, but can be bettered”. Pubmed
Amorelli, B., Yang, C., Rempel, B., Withers, S. G. and Knapp, S. (2008) Bioorg. Med. Chem. Lett. 18, 2944-7 “Synthesis and N-acetylhexosaminidase inhibitory properties of C-1 homologated GlcNAc- and GalNAc-thiazolines”. Pubmed
Wilkinson, S. M., Liew, C. W., Mackay, J. P., Saleh, H. M., Withers, S. G. and McLeod, M. D. (2008) Org. Lett. 10, 1585-8 “E. coli glucuronylsynthase: an engineered enzyme for the synthesis of glucuronides”. Pubmed
Rempel B. and Withers S. G. (2008) Glycobiology 18, 570-86 “Covalent inhibitors of glycosidases and their applications in biochemistry and biology”. Pubmed
Hekmat, O., He, S., Warren, R. A. J. and Withers, S. G. (2008) J. Prot. Res. 7, 3282-92 “A mechanism-based ICAT strategy for comparing relative expression and activity levels of glycosidases in biological systems”. Pubmed
Steiner, A. J., Stütz, A. E, Wrodnigg, T. M., Tarling, C. A., Withers, S. G., Hermetter, A. and Schmidinger, H. (2008) Bioorg. Med. Chem. Lett. 18, 1922-5 “Glycosidase profiling with immobilised glycosidase inhibiting iminoalditols: a proof-of-concept study”. Pubmed
Namdjou, D.-J., Chen, H., Vinogradov, E., Brochu, D., Withers, S. G. and Wakarchuk, W. W. (2008) Chembiochem 9, 1632-40 “A b-1,4-galactosyltransferase from Helicobacter pylori is an efficient and versatile biocatalyst displaying a novel activity for thioglycoside synthesis”. Pubmed
Damager, I., Buchini, S., Amaya, M. L., Buschiazzo, A., Alzari, P., Frasch, A. C., Watts, A. and Withers, S. G. (2008) Biochemistry 47, 3507-12 “Kinetic and mechanistic analysis of Trypanosoma cruzi trans-sialidase reveals a classical ping-pong mechanism with acid/base catalysis”. Pubmed
Zhang, R., McCarter, J. D., Braun, C., Wai, Y., Brayer, G. D. and Withers, S. G. (2008) J. Org. Chem. 73, 3070-7 “Synthesis and testing of 2-deoxy-2,2-dihalo glycosides as mechanism-based inhibitors of a-glycosidases”. Pubmed
Newstead, S., Potter, J. A., Wilson, J. C., Xu, G., Chien, C.-H., Watts, A., Withers, S. G. and Taylor, G. L. (2008) J. Biol. Chem. 283, 9080-8 “The structure of Clostridium perfringens Nan1 sialidase and its catalytic intermediates”. Pubmed
Lairson, L. L., Henrissat, B., Davies, G. J. and Withers, S. G. (2008) Ann. Rev. Biochem. 77, 521-55 “Glycosyltransferase: structures, functions and mechanisms”. Pubmed
Buchini, S., Buschiazzo, A. and Withers, S. G. (2008) Angew. Chemie 120, 2700-3 “Towards a new generation of specific Trypanosoma cruzi trans-sialidase inhibitors”. Pubmed
Maurus, R., Begum, A., Williams, L. K., Fredriksen, J. R., Zhang, R., Withers S. G. and Brayer, G. D. (2008) Biochemistry 47, 3332-44. “Alternative catalytic anions differentially modulate human a-amylase activity and specificity”. Pubmed
Shaikh, F. A. and Withers, S.G. (2008) Biochem. & Cell Biol. 86, 169-77 “Teaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesis”. Pubmed
Tarling, C. A., Woods, K., Zhang, R., Brastianos, H. C., Brayer, G. D., Andersen, R. J. and Withers, S. G. (2008) Chembiochem. 9, 433-8 “The search for novel human pancreatic a-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts”. Pubmed
Chen, H. M and Withers, S. G. (2007) Chembiochem. 8, 719-22 “Facile synthesis of 2,4-dinitrophenyl alpha-D-glycopyranosides as chromogenic substrates for alpha-glycosidases.” Pubmed
Hekmat, O., Florizone, C., Kim, Y-W., Eltis, L. D., Warren, R. A. J. and Withers, S. G. (2007) Chembiochem 8, 2125-32 “Specificity fingerprinting of retaining b-1,4-glycanases in Cellulomonas fimi secretome using two fluorescent mechanism-based probes”. Pubmed
Buchini, S. and Withers, S. G. (2007) Chimica Oggi 25, 1-4 “Engineered glycosidases for glycoside synthesis”.
Kim, Y.-W., Chen, H., Kim, J-H, Müllegger, J, Mahuran, D. and Withers, S. G. (2007) Chembiochem 8, 1495-9 “Thioglycoligase-based assembly of thio-disaccharides: screening as b-galactosidase inhibitors”. Pubmed
Hancock, S. M. and Withers, S. G. (2007) Encyclopedia of Life Sciences, John Wiley and Sons, 1-9 “Glycosidases: functions, families and folds”.
Meloncelli, P. J., Gloster, T. M., Money, V. A., Tarling, C. A., Davies, G. J., Withers, S. G. and Stick, R. V. (2007) Aust. J. Chem. 60, 549-565 “D-Glucosylated derivatives of isofagomine and noeuromycin and their potential as inhibitors of b-glycoside hydrolases”.
Shaikh, F. A., Muellegger, J., He, S. and Withers, S. G. (2007) FEBS Letters 581, 2441-6 “Identification of the catalytic nucleophile in Family 42 b-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis”. Pubmed
Jamaluddin, H., Tumbale, P., Withers, S. G., Acharya, K. R. and Brew, K. (2007) Journal of Molecular Biology 369, 1270-81 “Conformational changes induced by binding UDP-2F-galactose to a-1,3 galactosyltransferase – implications for catalysis”. Pubmed
Yip, V. L. Y., Thompson, J. and Withers, S. G. (2007) Biochemistry 46, 9840-52 “Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-a-glucosidase from glycoside hydrolase family 4″. Pubmed
Chen, H.-M. and Withers, S. G. (2007) Carbohydrate Research 342, 2212-22 “Syntheses of the 3- and 4-thio analogues of 4-nitrophenyl 2-acetamido-2-deoxy-b-gluco- and galactopyranoside”. Pubmed
Wicki, J., Schloegl, J., Tarling, C. A. and Withers, S.G. (2007) Biochemistry 46, 6996-7005 “Recruitment of both uniform and differential binding energy in enzymatic catalysis: xylanases from Families 10 and 11″. Pubmed
Schubert, M., Poon, D. K. Y., Wicki, J., Tarling, C. A., Kwan, E. M., Nielsen, J. E., Withers, S. G. and McIntosh, L. P. (2007) Biochemistry 46, 7383-95 “Probing electrostatic interactions along the reaction pathway of a glycoside hydrolase: Histidine characterization by NMR spectroscopy”. Pubmed
Steiner, A. J., Stuetz, A. E., Tarling, C. A., Withers, S. G. and Wrodnigg, T. M. (2007) Carbohydrate Research 342, 1850-8 “Iminoalditol-amino acid hybrids: synthesis and evaluation as glycosidase inhibitors”. Pubmed
Tropak, M. B., Blanchard J. E., Withers, S. G., Brown, E. D. and Mahuran, D. (2007) Chem. Biol. 14, 153-64 “High-throughput screening for human lysosomal b-N-Acetyl hexosaminidase inhibitors acting as pharmacological chaperones”. Pubmed
Caines, M. E. C., Hancock, S. M., Tarling, C. A., Wrodnigg, T. M., Stick, R. V., Stuetz, A. E., Vasella, A., Withers, S. G. and Strynadka, N. C. J. (2007) Angew. Chemie, Int. Ed. 46, 4474-6 “The structural basis of glycosidase inhibition by five-membered iminocyclitols: the clan A glycoside hydrolase endoglycoceramidase as a model system”. Pubmed
Wicki, J., Williams, S. J. and Withers, S.G. (2007) J. Am. Chem. Soc. 129, 4530-1 “Transition state mimicry by glycosidase inhibitors: a critical kinetic analysis”. Pubmed
Chiu, C. P. C., Lairson, L. L., Gilbert, M., Wakarchuk, W. W., Withers, S. G. and Strynadka, N. C. J. (2007) Biochemistry 46, 7196-7204 “Structural analysis of the a-2,3-sialyltransferase Cst-I from Campylobacter jejuni in both the apo and substrate-analogue bound forms”. Pubmed
Caines, M. E. C., Vaughan, M. D., Tarling, C. A., Hancock, S. M., Warren, R. A. J., Withers, S. G. and Strynadka, N. C. J. (2007) J. Biol. Chem. 282, 14300-8 “Structural and mechanistic analyses of endo-glycoceramidase II, a membrane-associated family 5 glycosidase in the apo and GM3 ganglioside-bound forms”. Pubmed
Hommalai, G., Withers, S. G., Chuenchor, W., Ketudat Cairns, J., R. and Svasti, J. (2007) Glycobiology 17, 744-53 “Enzymatic synthesis of cello-oligosaccharides by rice BGlu1 b-glucosidase glycosynthase mutants”. Pubmed
Poon, D. K. Y., Withers, S. G. and McIntosh, L. P. (2007) J. Biol. Chem. 282, 2091-2100 “NMR spectroscopic analyses reveal that the catalytic and cellulose-binding domains of Cellulomonas fimi b-(1,4)-glycosidase Cex are joined by a conformationally flexible glycosylated linker”. Pubmed
Lairson, L. L., Wakarchuk, W. W. and Withers, S. G. (2007) JCS Chem. Comm. 4, 365-7 “Alternative donor substrates for inverting and retaining glycosyltransferases”. Pubmed
Krupa, J. C., Shaya, D., Chi, L., Linhardt, R. J., Cygler, M., Withers, S. G. and Mort, J. S. (2007) Anal Biochem. 361, 218-25 “Quantitative continuous assay for hyaluronan synthase”. Pubmed
Poon, D. K. Y., Schubert, M., Au, J., Okon, M., Withers, S. G. and McIntosh, L. P. (2006) J. Am. Chem. Soc. 128, 15388-9 “Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H- 15N heteronuclear correlation spectroscopy”. Pubmed
Poon, D. K. Y., Ludwiczek, M. L., Schubert, M., Kwan, E. M., Withers, S. G. and McIntosh, L. P. (2007) Biochemistry 46, 1759-70 “Characterization of a b-(1,4)-Glycosidase along its reaction pathway: stabilization upon formation of the glycosyl-enzyme intermediate”. Pubmed
Lairson, L. L., Watts, A. G., Wakarchuk, W. W. and Withers, S. G. (2006) Nat. Chem. Biol. 2, 724-8 “Glycosyl transferase substrate engineering: harnessing substrate promiscuity and expanding biological catalysis through transient chemical modification”. Pubmed
Tesić, M., Wicki, J., Poon, D. K. Y., Withers, S. G. and Douglas, D. J. (2006) J. Am. Soc. Mass Spec. 18, 64-73 “Gas phase noncovalent protein complexes that retain solution binding properties: binding of xylobiose inhibitors to the b-1,4 exoglucanase from Cellulomonas fimi”. Pubmed
Yip, V. L. Y. and Withers, S. G. (2006) Angew. Chemie. 45, 6179-6182 “Family 4 glycosidases carry out efficient hydrolysis of thiodisaccharides via an a, b-elimination mechanism”. Pubmed
Kim, Y.-W., Chen, H., Kim, J-H and Withers, S. G. FEBS Letters 580, 4377-81 “Catalytic properties of a mutant b-galactosidase fromXanthomonas manihotis engineered to synthesize galactosyl-thio-b-1,3 and -b-1,4-glycosides”. Pubmed
Hancock, S. M., Vaughan, M. D. and Withers, S. G. (2006) Curr. Opin. Chem. Biol. 10, 509-19 “Engineering of glycosidases and glycosyltransferases”. Pubmed
Wrodnigg, T. M., Stütz, A. E., Tarling, C. A. and Withers, S. G. (2006) Carbohydr. Res. 341, 1717-22 “Fine-tuning of b-glucosidase inhibitory activity in the 2,5-dideoxy-imino-D-mannitol (DMDP) system”. Pubmed
Aharoni, A., Thieme, K., Chiu, C. P.C., Buchini, S., Lairson, L. L., Chen, H., Strynadka, N.C.J., Wakarchuk, W.W. and Withers, S.G. (2006) Nature Methods 3, 609-14 “High throughput screening of glycosyltransferase libraries: Evolution of a sialyltransferase with a novel substrate specificity”. Pubmed
Blair, D. E., Hekmat, O., Schuettelkopf, A. W., Shrestha, B., Tokuyasu, K., Withers, S. G. and van Aalten, D. M. F. Biochemistry 45, 9416-26 “Structure and mechanism of chitin deacetylase, a virulence factor from the fungal pathogen Colletotrichum lindemuthianum”. Pubmed
Mayer, C., Vocadlo, D. J., Mah, M., Rupitz, K. R., Warren, R. A. J. and Withers, S. G (2006) FEBS J. 273, 2929-41 “Characterization of a þ -N-acetylhexosaminidase and a bifunctional b-N-acetylglucosaminidase/ b-glucosidase from Cellulomonas fimi”. Pubmed
Gordon, R. D., Sivarajah, P., Satkunarajah, M., Ma, D., Tarling, C. A., Vizitiu, D., Withers, S. G. and Rini, J. M. (2006) J. Mol. Biol. 360, 67-79 “X-ray crystal structures of rabbit N -acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues”. Pubmed
Lemieux, M. J., Mark, B. L., Cherney, M., Withers, S. G., Mahuran, D. J. and James, M. N. G. (2006) J. Mol. Biol. 359, 913-29 “Crystallographic structure of human b-Hexosaminidase A: interpretation of Tay-Sachs mutations and loss of G M2 ganglioside hydrolysis”. Pubmed
Kim, Y-W., Fox, D. T., Hekmat, O., Kantner, T., McIntosh, L. P., Warren, R. A. J. and Withers, S. G. (2006) Org. Biomol. Chem. 4, 2025-32 “Glycosynthase-based synthesis of xylo-oligosaccharides using an engineered retaining xylanase from Cellulomonas fimi”. Pubmed
Pabba, J., Rempel, B. P., Withers, S. G. and Vasella, A. (2006) Helv. Chim. Acta. 89, 635-66 “Synthesis of glycaro-1,5-lactams and tetrahydrotetrazolopyrdine-5-carboxylates: inhibitors of b-D- glucuronidase and a-L-iduronidase”.
Müllegger, J., Chen, H., Chan, W. Y., Reid, S. P. Jahn, M., Warren, R. A. J., Salleh, H. M. and Withers, S. G. (2006) Chembiochem, 7, 1028-30 “Thermostable glycosynthases and thioglycoligases derived from Thermotoga maritima b-glucuronidase”. Pubmed
Vaughan, M. D., Johnson, K., DeFrees, S., Tang, X., Warren, R. A. J. and Withers, S. G. (2006) J. Am. Chem. Soc. 128, 6300-01 “Glycosynthase-mediated synthesis of glycosphingolipids”. Pubmed
Yip, V. L. Y. and Withers, S. G. (2006) Curr. Opin. Chem. Biol. 10, 147-55 “Breakdown of oligosaccharides by the process of elimination”. Pubmed
Kim, Y. W, Lovering, A. L., Chen, H., Kantner, T., McIntosh, L. P., Strynadka, N. C. J. and Withers, S. G. (2006) J. Am. Chem. Soc. 128, 2202-3 “Expanding the thioglycoligase strategy to the synthesis of a-linked thioglycosides allows structural investigation of the parent enzyme/substrate complex”. Pubmed
Mullegger, J., Chen, H., Warren, R. A. J. and Withers, S. G. (2006) Angew Chemie 45, 2585-8 “Glycosylation of a neo-glycoprotein using glycosynthase and thioglycosynthase approaches: the first generation of a thioglycoprotein”. Pubmed
Greimel, P., Hausler, H., Lundt, I., Rupitz, K., Stutz, A. E., Tarling, C. A., Withers, S. G. and Wrodnigg, T. M. (2006) Bioorg. Med. Chem. Lett. 16, 2067-70 “Fluorescent glycosidase inhibiting 1,5-dideoxy-1,5-iminoalditols”. Pubmed
Tauss, A., Steiner, A. J., Stutz, A. E., Tarling, C. A., Withers, S. G. and Wrodnigg, T. M. (2006) Tetrahedron Asymmetry 17, 234-9 “L-Idoseptanosides: substrates of glucosidases?”.
Rye, C.S., Matte, A, Cygler, M. and Withers, S.G. (2006) Chembiochem. 7, 631-37 “An atypical approach identifies Tyr234 as the key base catalyst in chondroitin AC lyase”. Pubmed
Yip, V. L. Y. and Withers, S. G. (2006) Biochemistry 45, 571-80 “Mechanistic analysis of the unusual redox-elimination sequence employed by BglT from Thermotoga maritime: a 6-phospho-b-glucosidase from glycosyl hydrolase Family 4″. Pubmed
Watts, A. G., Oppezzo, P., Withers, S. G., Alzari, P. M. and Buschiazzo, A. (2006) J. Biol. Chem. 281, 4149-55 “Structural and kinetic analysis of two covalent sialosyl-enzyme intermediates on Trypanosoma rangeli sialidase”. Pubmed
Mildvan, A. S., Xia, Z., Azurmendi, H. F., Legler, P. M., Balfour, M. R., Lairson, L. L., Withers, S. G., Gabelli, S. B., Bianchet, M. A. and Amzel, L. M. (2006) J. Mol. Struct. 790, 160-7 “Hydrogen bonding in the mechanism of GDP-mannose mannosyl hydrolase”.
Yip, V. L. Y. and Withers, S. G. (2006) Biocatalysis and Biotransformation 24, 167-76 “Family 4 glycoside hydrolases are special: The first b-elimination mechanism amongst glycoside hydrolases”.
Salleh, H. M., Mullegger, J., Reid, S., Chan, W. Y., Hwang, J., Warren, R. A. J. and Withers, S. G. (2006) Carbohydr. Res. 341, 49-59 “Cloning and characterization of Thermotoga maritima b-glucuronidase”. Pubmed
Lundt, I., Steiner, A., Stütz, A. E., Tarling, C., Ully, S. Withers, S.G. and Wrodnigg, T. M. (2006) Bioorg. Med. Chem. Letts. 14, 1737-42 “Fluorescently tagged iminoalditol glycosidase inhibitors as novel biological probes and diagnostics”. Pubmed
Williams, S. J., Hekmat, O. and Withers, S. G. (2006) Chembiochem 7, 116-24 “Synthesis and testing of mechanism-based protein profiling probes for retaining endo-glycosidases”. Pubmed